Is there any problem if my tile size and animated characters frame size is not equal? I have seen the rat example from another developer on this sub-forum so I know it works but my question is whether this would bring any negative effect to gameplay etc. Thanks for your feedback.

To be perfectly honest I'm not sure, what I would do is that if your animated character has a different framesize then the rest of your world is make sure that he has his own tileset as each tileset can have different frame sizes and that seems to all work fine.

As far as I'm aware it should be fine but this is what I need you guys for, to do all the experimentation that I don't have time for or don't even think of doing in the first place :-)

Sure thing. Being new to all this I just wanted to see if there is a best practice. I will report my experience. Thanks

All the tricks and techniques that you guys learn while in the Beta will become the best practices :-)